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KMID : 0358319970380020111
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Korean Journal of Urology
1997 Volume.38 No. 2 p.111 ~ p.119
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Proliferation Patterns of N-butyl-N-(4-hydroxybutyl) Nitrosamine(BBN) Induced Rat Bladder Tumor Identified by Histone MRNA In Situ Hydrdization.
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Abstract
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The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifity fold then rapidly disappears at the start of G2-phase. The presence, therefore, of
abundant
quantities of histone mRNa provides a molecular of cycling cells.
The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black
cytoplasmic
staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1). The higher S-phase fraction
indicated
greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
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